超声量化指标评价白藜芦醇对大鼠肝纤维化的影响

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摘要目的：通过超声量化指标观察白藜芦醇（Resveratrol，RES）对CCl4诱导的大鼠肝纤维化模型的影响。方法：20只健康雄性SD大鼠随机分为4组：对照组、RES组、模型组、RES干预组。造模8周后超声检测门静脉近段与远段内径、血流速度及肝组织杨氏模量，大鼠肝组织HE染色，光镜下判定肝纤维化程度。结果：①RES干预后大鼠症状较轻，体质量有所增加，肝纤维化病理得到改善。②大鼠门静脉近段与远段内径在模型组与RES干预组较对照组与RES组均增宽（P<0.05）；门静脉血流速度在模型组与RES干预组较对照组与RES组均增加，但在各组间比较差异均无统计学意义（P>0.05）；肝组织杨氏模量在模型组与RES干预组较对照组与RES组均升高（P<0.05）。RES干预后门静脉内径及杨氏模量改变幅度有所减低。各指标在对照组与RES组间比较差异均无统计学意义（P>0.05）。结论：①RES可以有效改善CCl4诱导的大鼠肝纤维化病理改变。②门静脉内径与杨氏模量可以有效评价RES对CCl4诱导大鼠肝纤维化的影响。

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	桑亮
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	袁胜美
	屠树星

关键词 ：肝硬化, 大鼠, 超声检查

Abstract：Objective: To investigate the effection of resveratrol(RES) on the CCl4-induced liver fibrosis in rat model by ultrasound quantitative index. Methods: Twenty male Sprague-Dawley rats were randomly divided into four groups: control group, RES group, model group, and RES intervention group. The proximal and distal diameter, blood flow velocity of portal vein and liver tissue Young’s modulus were detected by ultrasonography. The degree of hepatic fibrosis was determined under light microscope after HE stained. Results: ①After RES intervention, the symptoms of rats were mild with the weight increase, and the liver fibrosis was improved. ②In model and RES intervention group, the portal vein proximal and distal diameter, Young’s modulus of liver tissue were increased compared with control and RES group(P<0.05); The portal vein blood flow velocity was increased, but the differences among the groups were not statistically significant(P>0.05). The changes of the portal vein diameter and Young’s modulus were decreased after RES intervention. There were no significant differences between the control and RES group(P>0.05). Conclusion: ①RES can effectively improve the pathological changes of CCl4-induced liver fibrosis. ②Portal vein diameter and Young’s modulus can effectively evaluate the effect of RES on CCl4-induced liver fibrosis in rat.

Key words： Liver cirrhosis Rats Ultrasonography

收稿日期: 2016-09-20

PACS:	Ｒ657.31
	Ｒ445.1

通讯作者: 王学梅，中国医科大学附属第一医院超声科，110001。E-mail：wxmlmt@163.com

作者简介: 桑亮（1983-），男，山东寿光人，主治医师。E-mail：13889167622@163.com

引用本文:

桑亮，王学梅，许东阳，商功群，袁胜美，屠树星. 超声量化指标评价白藜芦醇对大鼠肝纤维化的影响[J]. 中国临床医学影像杂志, 2017, 28(4): 255-259.
SANG Liang, WANG Xue-mei, XU Dong-yang, SHANG Gong-qun, YUAN Sheng-mei, TU Shu-xing. Evaluation of the effect of resveratrol on liver fibrosis in rat by ultrasound quantitative index. JOURNAL OF CHINA MEDICAL IMAGING, 2017, 28(4): 255-259.

链接本文:

http://www.jccmi.com.cn/CN/ 或 http://www.jccmi.com.cn/CN/Y2017/V28/I4/255

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