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| MR tracking in vitro of adipose tissue-derived stem cells labeled#br#
with self-synthesis superparamagnetic iron oxide nanoparticles |
| The First Affiliated Hospital of Dalian Medical University, Dalian Liaoning 116011, China |
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Abstract Objective: To assess the effects of superparamagnetic iron oxide(SPIO) nanoparticles on viability and function of adipose tissue-derived stem cells(ADSCs), and characterize the magnetic properties of the SPIO-labeled ADSCs in vitro. Methods: The silicon and amine coated SPIO(Fe3O4) nanoparticles were donated by Dalian Institute of Chemical Physics. ADSCs were obtained and cultured from adipose tissue of patients who had a mastectomy under informed consent. The SPIOs label was added to the cultures at Fe concentrations equivalent to 1, 5, 10, 25, 50, 100 μg/mL and incubated for 24 h. Control cultures were incubated without SPIOs. The labeling efficiency was calculated as number of Prussian blue labeled cells/total number of cells in the sample. MTT was used for cell viability assay. ADSCs were incubated with SPIO at 25 μg/mL for 24 h. T1WI, T2WI, T2*WI and R2* mapping images were acquired using a 3.0T-MR scanner(GE signal HDxt, USA), values and change rate of T1WI, T2WI, T2*WI and R2* were measured. Results: We observed a good correlation between SPIO uptake and the iron concentration in the culture medium. As the concentration of iron increased, the amount of intracellular iron increased, but there is no significant difference when the concentration was greater than 25 μg/mL. Treatment with doses up to 25 μg/mL significantly affected the survival and proliferation of ADSCs. At the same time values of T2*WI and R2* rise obviously. Conclusion: The amine-surface-modified SPIO nanoparticles are not deleterious to ADSCs and do not affect their proliferation, and which appeared to be a candidate for in vitro ADSCs labeling. The optimum concentration is 25 μg/mL.
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