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| Preliminary study on assessment of regadenoson-induced blood-brain barrier opening by MR perfusion weighted imaging |
| CHANG Can-can1, ZHOU Jun2, YANG Ben-qiang1, LI Hong-yi1, XU Zhi-hua1, LIN Sen1, DUAN Yang1 |
| 1. Department of Radiology, the General Hospital of Shenyang Military Region, Shenyang 110016, China;#br# 2. Department of Radiology, Shenyang the Fourth Hospital of People, Shenyang 110031, China |
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Abstract Objective: To evaluate the level of regadenoson-induced blood-brain barrier opening of healthy rabbits via MR perfusion weighted imaging(MR PWI) scan. Methods: Fifty New Zealand white rabbits were randomly divided into experimental group(group A) and control group(group B). The regadenoson and normal saline were injected intravenously into ear margin separately, after 10 minute, MR PWI scan was performed. Then rCBF, rCBV, TTP and MTT of the region of interest of each group were acquired and compared statistically(paired t test). Five cases of each group choosed randomly were injected with 2% Evens blue intravenously posterior to imaging. After one hour of infusion, all the animals were killed. Their brains were examined for the determination of Evens blue distribution. Results: The rCBF and rCBV values and the staining with Evens blue of group A were significantly higher than those of group B(P<0.05). There were no significant differences of TTP and MTT values between group A and B(P>0.05). Conclusion: The parameters(rCBF, rCBV) of MR PWI scan can monitor the level of regadenoson-induced blood-brain barrier opening in vivo.
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Received: 07 January 2016
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[1]Daneman R, Barres BA. The blood-brain barrier-lessons from moody flies[J]. Cell, 2005, 123(1): 9-12.
[2]Agarwal V, DePuey EG. Regadenoson and seizures: a real clinical concern[J]. J Nucl Cardiol, 2014, 21(5): 869-870.
[3]Rajadhyaksha M, Boyden T, Liras J, et al. Current advances in delivery of biotherapeutics across the blood-brain barrier[J]. Curr Drug Discov Technol, 2011, 8(2): 87-101.
[4]Abbott NJ, Patabendige AA, Dolman DE, et al. Structure and function of the blood-brain barrier[J]. Neurobiol Dis, 2010, 37(1): 13-25.
[5]Hossain S, Akaike T, Chowdhury EH. Current approaches for drug delivery to central nervous system[J]. Curr Drug Deliv, 2010, 7(5): 389-397.
[6]Pardridge WM. The blood-brain barrier: bottleneck in brain drug development[J]. NeuroRx, 2005, 2(1): 3-14.
[7]Marchi N, Angelov L, Masaryk T, et al. Seizure-promoting effect of blood-brain barrier disruption[J]. Epilepsia, 2007, 48(4): 732-742.
[8]Cook AM, Mieure KD, Owen RD, et al. Intracerebroventricular administration of drugs[J]. Pharmacotherapy, 2009, 29(7): 832-845.
[9]Jenne JW. Non-invasive transcranial brain ablation with high-intensity focused ultrasound[J]. Front Neurol Neurosci, 2015, 36: 94-105.
[10]Yu YJ, Atwal JK, Zhang Y, et al. Therapeutic bispecific antibodies cross the blood-brain barrier in nonhuman primates[J]. Sci Transl Med, 2014, 6(261): 261.
[11]Witt KA, Gillespie TJ, Huber JD, et al. Peptide drug modifications to enhance bioavailability and blood-brain barrier permeability[J]. Peptides, 2001, 22(12): 2329-2343.
[12]赵博贤，毕云科，李宪峰，等. 腺苷受体与血脑屏障通透性研究进展[J]. 临床神经外科杂志，2013，10（4）：248-250.
[13]Prasain N, Stevens T. The actin cytoskeleton in endothelial cell phenotypes[J]. Microvasc Res, 2009, 77(1): 53-63.
[14]Carman AJ, Mills JH, Krenz A, et al. Adenosine receptor signaling modulates permeability of the blood-brain barrier[J]. J Neurosci, 2011, 31(37): 13272-13280.
[15]Villringer A, Rosen BR, Belliveau JW, et al. Dynamic imaging with lanthanide chelates in normal brain: contrast due to magnetic susceptibility effects[J]. Magn Reson Med, 1988, 6(2): 164-174.
[16]Provenzale JM, Wang GR, Brenner T, et al. Comparison of permeability in high-grade and low-grade brain tumors using dynamic susceptibility contrast MR imaging[J]. Am J Roentgenol, 2002, 178(3): 711-716.
[17]Morita N, Wang S, Chawla S, et al. Dynamic susceptibility contrast perfusion weighted imaging in grading of nonenhancing astrocytomas[J]. J Magn Reson Imaging, 2010, 32(4): 803-808.
[18]周丽，付旷，郭丽丽，等. 3.0T磁共振灌注加权成像在脑胶质瘤诊断中的价值[J]. 中国实验诊断学，2013，17（2）：275-278.
[19]陆娜，冯晓源，邸悦，等. CT灌注成像评价人脑胶质瘤肿瘤血管生成[J]. 中国医学计算机成像杂志，2014，20（6）：481-484. |
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