水通道蛋白磁共振分子成像与水通道蛋白4表达的相关性研究

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Abstract Objective: To explore the correlation between aquaporin magnetic resonance molecular imaging(AQP MRI) and the expression of AQP4. Methods: Ten adult SD male rats were subjected to either predisposing(LRP) or sham surgery and then subsequently underwent right middle cerebral artery occlusion(MCAo) for one hour. AQP MRI was executed 48 h after reperfusion. And the AQP4 expression was detected by Western blot. Results: The success rate of modeling was 60%, meaning there were only 6 rats living up to the criteria of modeling. AQP MRI showed AQP ADC values of the affected side of rats No.1~5 were lower than those of the uninjured side, but the AQP ADC values in the affected side of rat No.6 was much higher than that of the uninjured side. Western blot results showed that the AQP4 expression in the affected side of No.1~6 rats was higher than that of the uninjured side. Correlation analysis showed that the AQP ADC values were negatively correlated to the level of AQP4 protein expression when excluding rat No.6. Conclusion: AQP MRI can display the expression quantity as well as the distribution of AQP4 in vivo, and it has great value in clinical application.

Key words： Brain ischemia Magnetic resonance imaging

Received: 05 April 2016

PACS:	Ｒ743
	Ｒ445.2

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	Articles by authors
	CHEN Qiu-yan
	WU Fu-lin
	PENG Xiao-lan
	LI Chun-li
	JIANG Min
	CHEN Ting-ting
	WEI Ding-tai

Cite this article:

CHEN Qiu-yan,WU Fu-lin,PENG Xiao-lan, et al. Research on correlation between aquaporin magnetic resonance molecular imaging and AQP4 expression[J]. JOURNAL OF CHINA MEDICAL IMAGING, 2016, 27(12): 837-841.

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http://www.jccmi.com.cn/EN/ OR http://www.jccmi.com.cn/EN/Y2016/V27/I12/837

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