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| The effects of NF-κB antisense RNA gene on the levels of serum TNF-α and IL-1β in rheumatoid arthritis rats by ultrasound-mediated microbubble destruction |
| LIU Jie, JING Xiang-xiang, YANG Bing-ang, FU Shao-qing, WANG Dong-lin, WU Tang-na |
| Hainan Provincial People’s Hospital, Haikou 570311, China |
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Abstract Objective: To investigate the effect of NF-κB antisense RNA gene delivery mediated by ultrasound-mediated microbubble destruction on serum levels of TNF-α, IL-1β in rheumatoid arthritis(RA) rats and its feasibility for RA therapy. Methods: The layer-by-layer assembly technique was applied to attach multiple layers of gene and polyllysine onto prepared lipid-coated microbubbles(PLL+DNA+PLL Apply+DNA)． Sixty rats were divided into six groups(10 rats with 20 knees in each group). Group A: normal control group; group B: blank control group of RA rats; group C: NF-κB antisense RNA gene plasmid group; group D: ultrasound+NF-κB antisense RNA gene plasmid group; group E: microbubbles+NF-κB antisense RNA gene plasmid group; group F: ultrasound+microbubbles+NF-κB antisense RNA gene plasmid group. The serum levels of TNF-α, IL-1β in RA rats were detected with ELISA. Results: The levels of serum TNF-α and IL-1β were obviously higher than normal control group. Those of group C to F were notably lower than that of the RA control group(P＜0.05, P＜0.01). There was no statistical difference in the levels of serum TNF-α and IL-1β among group C, D and E. But there was statistics difference between group F and group C to E(P＞0.05). Conclusion: NF-κB antisense RNA gene transfection efficiency on RA rat’s joints synovial tissues can be enhanced by ultrasound-mediated microbubble destruction, which can down-regulate the levels of serum TNF-α and IL-1β, and enhance the therapeutic effect of RA.
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Received: 28 March 2016
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LIU Jie,JING Xiang-xiang,YANG Bing-ang, et al. The effects of NF-κB antisense RNA gene on the levels of serum TNF-α and IL-1β in rheumatoid arthritis rats by ultrasound-mediated microbubble destruction[J]. JOURNAL OF CHINA MEDICAL IMAGING, 2016, 27(12): 896-898.
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| http://www.jccmi.com.cn/EN/ OR http://www.jccmi.com.cn/EN/Y2016/V27/I12/896 |
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