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| Correlation of CaSR986 gene polymorphism with clinical characteristics and urinary calculi composition by energy spectrum CT |
| LI Qin-xiang, PAN Ai-zhen, XU Zhi-feng, JIN Ya-bin, LIU Wei-tao, XU Wen-feng |
| The First People’s Hospital of Foshan, Foshan Guangdong 528000, China |
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Abstract Objective: To explore the correlation of CaSR986 gene polymorphism with clinical characteristics and urinary calculi composition by the spectrum CT. Methods: Ninety-eight patients with urinary calculi admitted to our hospital from January 2016 to July 2017 were enrolled in the experimental group, and 50 healthy people in our hospital during the same period were collected as the control group. The genotype and allele composition of CaSR986 in the two groups were analyzed by polymerase chain reaction and DNA sequencing, and meanwhile the gemstone spectrum CT scan was used to analyze the composition in the experimental group before operation(using postoperative infrared spectroscopy as a standard, excluding the differences in the results). The recurrence and composition of stones in the experimental group were recorded with one year follow-up. The correlation between the distribution of each genotype of CaSR986, the stone composition and clinical features was analyzed. The independent risk factors of stone recurrence were analyzed by Logistic regression. Results: The distribution of CaSR986 gene in the experimental group and control group was in accordance with Hardy-Weinberg law, and there was no significant difference in genotype and allele composition between the two groups(P>0.05). Seventy-five cases of calcium stone and 23 cases of non-calcium stone were found in the experimental group by HDCT and FTIR automatic analysis. The pearson correlation analysis showed that calcium-containing stones were positively correlated with CaSR986 AA(r=0.734, P<0.05). However, there was no significant correlation between calcium-containing stones and other genotypes of CaSR986, non-calcium-containing stones and any genotype of CaSR986(P>0.05). CaSR986 genotype was significantly different in stone location, recurrence and recurrence components(P<0.05), but there was no significant difference in stone diameter or hydronephrosis(P>0.05).The Logistic regression analysis showed that stone composition was an independent risk factor for urinary calculi recurrence(P <0.05). Conclusion: Gemstone spectrum CT can distinguish calculus before operation which is valuable in predicting recurrence of urinary calculi and is closely related to CaSR986AA genotype.
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Received: 13 September 2018
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2020, Vol. 31 
