Validity and repeatability of CMR GRE for evaluating the heart function of rhesus monkey
ZHU Tong1, GONG Li2, CHEN Yu-shu1, ZHANG Yu1, LIANG Zhi-gang2, SUN Jia-yu1, #br#
CHENG Wei1, WANG Lei1, WU Bing1, ZHENG Jie3, ZENG Wen2, GAO Fa-bao1
1. Department of Radiology, West China Hospital, Sichuan University, Chengdu 610000, China;
2. Sichuan Primed Biotech Group Co., LTD, Chengdu 610000, China;
3. Mallinckrodt Institute of Radiology, Washington University School of Medicine in St. Louis, MO, 63101, USA
Abstract:Objective: To evaluate the validity and repeatability of GRE cine sequence when it was used to evaluate the heart function of rhesus monkey, and to provide an accurate and optimal cardiac function assessment strategy for the study of rhesus monkey cardiac disease models. Methods: Eight healthy rhesus monkeys underwent conventional echocardiography and CMR GRE cine sequence scanning to assess cardiac function and got left ventricular end-diastolic volume(EDV), left ventricular end-systolic volume(ESV), stroke volume(SV), ejection fraction(EF), cardiac output(CO), left ventricular end-diastolic mass (Myo Mass(Diast)) and left ventricular end-systolic mass(Myo Mass(Syst)). The repeatability of GRE was evaluated by the test-retest analysis. The validity of the GRE was evaluated by criterion-related validity by comparing the results of ultrasound and GRE. Results: The results of test-retest analysis showed that correlation coefficient(ICC) values of all function parameters were within 0.75~0.97, the P value was less than 0.01, and the results of repeated evaluation were better. The results of criterion-related validity showed that except for CO, the Pearson correlation coefficient(r) values of the other function parameters were 0.80~0.92, and the difference was statistically significant. It was found that EF, CO and Myo Mass(Diast) acquired by GRE were smaller than those measured by ultrasound, and the difference was statistically significant. Conclusion: There are good validity and repeatability using the CMR GRE cine sequence to evaluate the cardiac function of normal rhesus monkey.
朱 桐1,龚 立2,陈榆舒1,张 钰1,梁志刚2,孙家瑜1,程 巍1,王 磊1,伍 兵1,郑 捷3,曾 文2,郜发宝1. 磁共振GRE电影序列评价恒河猴心功能的重复性及有效性研究[J]. 中国临床医学影像杂志, 2017, 28(10): 727-730.
ZHU Tong1, GONG Li2, CHEN Yu-shu1, ZHANG Yu1, LIANG Zhi-gang2, SUN Jia-yu1, CHENG Wei1, WANG Lei1, WU Bing1, ZHENG Jie3, ZENG Wen2, GAO Fa-bao1. Validity and repeatability of CMR GRE for evaluating the heart function of rhesus monkey. JOURNAL OF CHINA MEDICAL IMAGING, 2017, 28(10): 727-730.
[1]Lorenz CH, Walker ES, Morgan VL, et al. Normal human right and leftventricular mass, systolic function, and gender differences by cine magnetic resonance imaging[J]. J Cardiovasc Magn Reson, 1999, 1(1): 7-21.
[2]Salerno M, Kramer CM. Advances in parametric mapping with CMR imaging[J]. JACC Cardiovasc Imaging, 2013, 6(7): 806-822.
[3]Walton S, Berger K, Thiyagalingam J, et al. Visualizing Cardiovascular Magnetic Resonance(CMR) imagery: challenges and opportunities[J]. Prog Biophys Mol Biol, 2014, 115(2-3): 349-358.
[4]Anon. Demands for rhesus monkeys in biomedical research: a workshop report[J]. ILAR, 2003, 44(3): 222-235.
[5]Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement[J]. Lancet, 1986, 1(8476): 307-310.
[6]Koo TK, Li MY. A Guideline of Selecting and Reporting Intraclass Correlation Coefficients for Reliability Research[J]. J Chiropr Med, 2016, 15(2): 155-163.
[7]Rusy B, Komai H. Anesthetic depression of myocardial contractility: a review of possible mechanisms[J]. Anesthesiology, 1987, 67(5): 745-766.
[8]Zamorano JL, Bax JJ, Rademakers FE, et al. Evaluation of Systolic and Diastolic LV Function. The ESC Textbook of Cardiovascular Imaging[M]. New York: Springer, 2010: 307-320.
[9]Childs H, Ma L, Ma M, et al. Comparison of long and short axis quantification of left ventricular volume parameters by cardiovascular magnetic resonance, with ex-vivo validation[J]. J Cardiovasc Magn Reson, 2011, 13(1): 40.