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| CT perfusion C6 cerebral gliomas in rat |
| GUAN Li-ming, XU Ke, GUO Min, QI Xi-xun, FANG Chang-xing, LIU Shu-li |
| First Affiliated Hospital, China Medical University, Shenyang 110001, China |
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Abstract Objective: To evaluate growth and angiogenesis of C6 cerebral gliomas in rat by CT. Methods: C6 cells were inoculated into the right caudate nucleus of 30 Wistar rats by stereotactic procedure to established animal model. CT perfusion was performed at 1 week, 2 and 3 weeks after implantation. Immediately after each examination, some rats were sacrificed for pathology. Results: Although there was no angiogenesis found, all the values of perfusion parameters for the tumor at 1 week after implantation increased compared to the contralateral side(P<0.05). The values of CBF, CBV, PS in the tumor and PS in the tumor-brain interface at 2 and 3 weeks after implantation were higher than that in the regions of contralateral brain, only the PS value had statistical difference in comparing the center of the tumor with the periphery of the tumor and the tumor-brain interface. The microvascular density(MVD) within the tumor, especially at the periphery of the tumor, increased significantly compared to the contralateral side(P<0.05). The perfusion volume and permeability increased with growth of the tumor during 3 weeks after implantation, in which positive correlation was present not only between CBV and MVD in the center and at the periphery of the tumor but also PS and MVD in the center of the tumor(rs=0.576, 0.482, 0.646 respectively, P<0.05). Conclusion: The parameters of CT perfusion can evaluate tumor microcirculation with the development of rat C6 brain gliomas in vivo, while CBV and PS, change obviously at the periphery of the tumor, can be the more sensitive index reflecting tumoral angiogenesis.
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Received: 10 August 2007
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Corresponding Authors:
XU Ke
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2007, Vol. 18 
