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| Control study of CT perfusion imaging and histology of rabbit liver VX2 tumor before and after the application of Sorafenib |
| WU Yue1, LIANG Chang-hong1, YANG Ben-qiang2, LIU Wen-yuan2, LI Zi-hao2 |
| 1. Department of Radiology, Guangdong Provincial People’s Hospital, Guangdong Provincial Academy of Medical Science, Guangzhou 510080, China; 2. Department of Radiology, General Hospital of Shenyang Military Command, Shenyang 110016, China |
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Abstract Objective: To study the CT perfusion parameter change and histology of rabbit liver VX2 tumor before and after the application of Sorafenib, it was supposed to explore the feasibility of CT perfusion imaging in monitoring of the growth of liver tumor, and to provide experimental basis for evaluation the effect of Sorafenib inhibiting tumor angiogenesis and promoting cell apoptosis. Methods: The multi-slice CT perfusion was performed in 12 New Zealand white rabbits at 2 weeks after VX2 hepatic tumor implantation. Two were executed as the control group of pathology. The various perfusion parameters(HBF, HBV, MTT, PS, HAF) were measured in the tumor area and non-tumorous regions in other 10 rabbits． Sorafenib was administered by mouth at the dosage of 20mg/kg/d for one week. The second CT perfusion was performed at 3 weeks, the parameters of tumor region were compared by the previous result. After that, all the rabbits were executed and pathological examination and Caspase-3 staining was performed. Result: Twelve(100%) rabbits were successfully implanted with VX2 tumor in the liver． It was demonstrated that the increased HBF, HBV, PS, HAF and the decreased MTT in the tumor area were differed significantly from the non-tumorous regions at 2 weeks after VX2 hepatic tumor implantation(P<0．01). The statistical analysis also showed significant difference in HBF, PS, HAF of liver tumor at the two CT perfusion(P<0．01). And no significant difference in HBV, MTT. The expression intensity of Caspase-3 was significantly lower than that of normal control group(P<0．05)． Conclusion: The multi-slice CT perfusion imaging had advantages in the changes of hemodynamics of the rabbit model of VX2 liver tumor, the effect of Sorafenib inhibiting tumor angiogenesis and promoting cell apoptosis could be more identified if combination with the research of histology.
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Received: 17 March 2010
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Corresponding Authors:
LIANG Chang-hong
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2010, Vol. 21 
