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| Discussion of Gd-EOB-DTPA dynamic enhanced MRI scan in normal liver of mice |
1. Affiliated Hospital of Military Medical Science Academy of the PLA, Beijing 100071, China;
2. Institute of Disease Control and Prevention, Military Medical Science Academy of the PLA, Beijing 100071, China |
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Abstract Objective: To investigate the optimal imaging parameters of MRI T1 plain scan and Gd-EOB-DTPA dynamic enhanced scan in kunming mice based on small animal experiments on conventional MRI, to provide references for the experimental study of small animal liver imaging. Methods: Ten male kunming mice(6~8 W) were performed with T1WI and T1 dynamic enhanced scanning(10-phase dynamic enhanced continuous scanning) using Siemens 3.0T MRI and special coil of wrist joint and tail vein injection. Gd-EOB-DTPA was diluted 100 times with saline. Dosage: 0.05 mL/10g, 0.1 mL/10g, 0.15 mL/10g. The time and signal intensity percentage curve was formed after T1WI plain scan and dynamic enhancement, and the shape of the curve was observed. The T1WI signal intensity of the liver, the signal intensity of the liver at the time of the development of the gallbladder and the tissue contrast of the liver and muscle signal were measured and analyzed. The enhancement rates of the liver parenchyma signal were calculated and compared with the muscle signal enhancement rates. Results: The dose of tail vein injection contrast agent was 0.1 mL/10g, and the time and signal intensity percentage curve was rising platform type, and followed by the platform type after the fourth stage, and the gallbladder developed at fifth or sixth stage. There were significant differences of the signal intensity between plain scans and enhanced scans in small animal livers, and of the signal enhancement rate between the liver parenchyma and the muscle. The contrast of the plain scan signal intensity of the liver to the muscle was significantly different from the contrast of the enhanced signal intensity of the liver. Conclusion: Small animal experiments on conventional MRI were feasible. The Gd-EOB-DTPA dynamic enhanced scan in kunming mice was also feasible. The curve of the Gd-EOB-DTPA dynamic enhanced scan showed rising platform type. The developing of gallbladder was clear. The Gd-EOB-DTPA can be used for the research of the related liver diseases.
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Received: 04 May 2015
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