Ⅰ~Ⅱ期糖尿病肾病大鼠模型扩散张量成像的初步研究

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Abstract Objective: To prospectively assess the manifestation of stage Ⅰ and Ⅱ diabetic nephropathy rats using diffusion tensor imaging(DTI) MRI at 3.0T. Materials and Methods: Twenty male SD rats were randomly divided into 2 groups according to the random number table. Fourteen rats were in the experimental group, the final survival of 9 rats with 18 kidneys, and 6 rats were in the control group with 12 kidneys. The experimental group was treated with 1% streptozotocin, and the control group was given the same dose of citrate buffer solution. Two weeks later, both of the two groups of rats underwent conventional MRI and DTI examination with b value of 0 and 600 s/mm2(diffusion directions=6), and the serum creatinine and microalbuminuria were measured and the pathological change of all kidneys were examined. Mean apparent diffusion coefficient(ADC) and fractional anisotropy(FA) were determined for the cortex and the medulla by two experienced radiologists blinded. Results: The blood glucose of experimental group was significantly increased, and the serum creatinine and microalbuminuria in both of the two groups of rats were normal. The pathological changes of the kidneys in the experimental group were stage Ⅰ and Ⅱ diabetic nephropathy expression, and the control group was normal. The ADC values of cortex and medulla were increased in the experimental group compared with the control group and the difference was statistically significant(P<0.05). In the experimental group, the difference of FA values were statistically significant between cortex and medulla(P<0.05). While there were no significant differences in the FA values of the cortex and medulla between two groups(P>0.05). Conclusion: The ADC value and FA value of DTI imaging maybe be more able to reflect the pathological changes than the biochemical indicator in the early stage of diabetic nephropathy.

Key words： Diabetic nephropathies Magnetic resonance imaging

Received: 05 April 2016

PACS:	Ｒ587.2
	Ｒ445.2

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	Articles by authors
	HU Xiao-yan1
	WU Ying-hua1
	GUO Liang2
	KUANG Min1
	WANG Fan3

Cite this article:

HU Xiao-yan1,WU Ying-hua1,GUO Liang2, et al. A preliminary study in stage Ⅰ and Ⅱ of diabetic nephropathy rats using diffusion tensor imaging MRI at 3.0T[J]. JOURNAL OF CHINA MEDICAL IMAGING, 2016, 27(11): 795-798.

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http://www.jccmi.com.cn/EN/ OR http://www.jccmi.com.cn/EN/Y2016/V27/I11/795

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[1]	GU Chun-hui, ZHENG Li-ping. [J]. JOURNAL OF CHINA MEDICAL IMAGING, 2016, 27(9): 681-681.