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| Binding 18F-FDG and 18F-fluoroacetate Micro-PET imaging in human HCC model |
1. The Hainan Branch of the PLA General Hospital, Sanya Hainan 572013, China;
2. The PLA General Hospital, Beijing 100853, China |
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Abstract Objective: The aim of study was to evaluate whether the 18F-FDG and 18F-fluoroacetate(18F-FAC) could be used to distinguish the different metastatic potential of hepatocellular carcinoma(HCC) models. Methods: Human HCC cell lines HepG2 and QGY7701 were cultured and implanted into nude mice to create HCC models. In vitro cell invasion assay was performed to judge invasion ability. Uptakes of 18F-FDG, 18F-FAC in two cells were assessed by in vitro uptake assay. In vivo imaging of tumor-bearing mice were obtained by animal PET/CT after injection of each tracer. ROI(region of interest) was drawn over tumor and lung to calculate T/NT(tumor/non-tumor). Results: The invasion ability of HepG2 was much higher than QGY7701(P<0.05), and the uptake ratio of 18F-FDG and 18F-FAC in HepG2 was higher than that in QGY7701(P<0.05) in vitro uptake assay. The 18F-FDG uptake of cells correlated with invasion capability(r=0.657, P<0.05). Meanwhile, in Micro-PET/CT study, the uptake of 18F-FDG and 18F-FAC in HepG2 tumor was higher than that in QGY7701 tumor(P<0.05). Conclusion: High uptake of 18F-FDG reflects higher invasion ability of HCC cell. Meanwhile, there is differentiation between two HCC models in 18F-FDG Micro-PET imaging. It indicates that 18F-FDG PET imaging could help to predict metastasis ability of HCC.
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Received: 31 August 2015
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