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| Experimental study of multi-b value DWI to evaluate efficacy of#br# endostar in human colon cancer xenograft in nude mice |
| Department of Radiology, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, China |
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Abstract Objective: To discuss the value of multi-b value DWI in evaluating efficacy of endostar in the human colon carcinoma xenograft tumor in nude mice non-invasively. Methods: HT-29 human colon cancer cells were seeded in 48 nude mice. The 48 mice were randomly divided into two groups, the control group and the experimental group, with 24 mice in each. Multi-b value DWI was acquired before, 2 days, 7 days and 14 days after treatment. Change of apparent diffusion coefficient(ADC) was observed. Eight nude mice were sacrificed after scanning 2 days, 7 days and 14 days after treatment, and immunohistochemical examination was done. Results: After treatment, ADC10b and ADChigh values of the tumor in endostar group increased gradually. ADClow and ADCperf values of the tumor in endostar group decreased gradually. There was statistically significant difference among four groups at different time points before and after treatment(F=29.986, 23.742, 21.598, 63.973, P＜0.05). In a paired comparison between time points, ADC10b, ADChigh and ADCperf were statistically different before, 2 days, 7 days and 14 days after treatment. ADClow was statistically different between different time points, but not between 2 days and 7 days after treatment. The other indexes of the tumor were statistically different in the comparison of every two time points. In the experimental group, the ADCperf value was positively correlated with microvessel density(MVD) at three time points(P＜0.05) and the ADC10b value was negatively correlated with MVD(P＜0.05). Conclusion: Multi-b value DWI can evaluate dynamically the change of human colon tumor xenograft model in nude mice after drug treatment, which can be considered as a reference index of early anti-angiogenesis drug efficacy.
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Received: 22 September 2015
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Corresponding Authors:
JIA Wen-xiao
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