|
|
|
| Study of the feasibility of a novel, clinical applicable cell labeling method to trace the transplanted cells in vivo |
| HE Geng-xu1, YAO Tong2, ZHANG Hao3, HU Sheng-shou3, SONG Peng3, ZHANG Xiao-ling3 |
| 1． Department of Thoracic and Cardiovascular Surgery, the 251st Hospital of PLA, Zhangjiakou Hebei 075000, China; 2． First Affiliated Hospital of North College, Zhangjiakou Hebei 075000, China; 3． Cardiovascular Institute and Fuwai Hospital, Beijing Union Medical College & Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, Beijing 100037, China |
|
|
|
|
Abstract Objective: To find a new labeling agent to trace and visualize the transplanted cells in vivo. Methods: Bone marrow MSCs were incubated with SPIO for 36 hours. The labeling efficiency was tested through Prussian Blue staining, the growth ability was evaluated through MTT, and cells viability was tested through Trypan Blue rejection method, the migratory ability was assessed with Costar Transwell plates, the differentiation of labeled cells to adipogenic and osteogenic cells was induces with induction medium. The labeled or unlabeled cells were transplanted into the myocardium or coronary artery. MRI was carried out immediately and 1～4 weeks respectively. After MRI the heart was excised, the segment in which injections performed were thin cut and stained with hematoxylin-eosin and Prussian Blue staining. Results: There were intracytoplasmic blue particles in nearly every cell in Prussian Blue staining. SPIO had no poison effect on the cell growth and proliferation. The viability of cells was more than 98%. The migratory ability was not affected. The labeled MSCs could still be differentiated from adipogenic and osteogenic cells after being incubated in the inductive medium. The labeled cells could be detected through MRI and confirmed by pathology. After 4 weeks the injected labeled cells could still be detected through MRI. The pathology showed the injected cells could survive in the MI area, and parallel in the same direction. Conclusion: The cells could be efficiently and safely labeled with SPIO and the labeled cells could be reliably detected by MRI in vivo.
|
|
Received: 10 October 2008
|
|
|
|
Corresponding Authors:
HU Sheng-shou
|
|
|
|
|
|
|
2009, Vol. 20 
