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| Study on the correlation between ultra-high-b-value diffusion-weighted MR imaging and #br#
aquaporin-4 in a hepatic ischemia-reperfusion model in rats |
| ZHANG Si-ying1, YAO Lin-peng1, WANG Qi-dong1, HONG Yuan2, XUE Xing1, KONG De-xing2, CHEN Feng1 |
1. Department of Radiology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;
2. School of Mathematical Science, Zhejiang University, Hangzhou 310003, China |
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Abstract Objective: To study the expression of aquaporin-4(AQP-4) in hepatic ischemia-reperfusion tissue, and to explore the correlation between ultra-high-b-value magnetic resonance diffusion-weighted imaging(DWI) and the expression of AQP-4. Methods: Forty-two SD rats weighting 250～300 g were divided into 7 groups(n=6). The rats in the experimental group were treated with ligating the right lobe portal vein and hepatic artery for 3 h. The rats in the control group were treated with sham operation. 3.0T MRI scanning including plain scan T1WI, T2WI, conventional DWI(b=0 s/mm2, 1 000 s/mm2) and 15 b-values DWI(b=0～4 500 s/mm2) were performed in both groups at 0 h, 6 h, 12 h, 1 d, 3 d, 7 d and 14 d after reperfusion. Standard apparent diffusion coefficient(ADCs) and ultra-high apparent diffusion coefficient(ADCu) were measured based on single b-value DWI and 15 b-values DWI respectively. The dynamic changes of each parameter were observed at each time point. After scanning, the animals were euthanized and liver specimens were taken for HE staining. The morphological changes of the liver were noted under light microscope. The expression of AQP-4 was measured qualitatively and quantitatively by Western blot. Results: The ADCs in the experimental group were higher than those of the control group starting from 12 hours after operation and returned to normal at 7 d after operation. ADCu in the experimental group began to significantly higher than those in the control group at 6 h after operation and returned to normal at 14 d after operation. The expression of AQP-4 in the experimental group decreased gradually at 6 h, 12 h, 1 d and 3 d after operation but still higher than that in the control group and began to approach normal at 7 d after operation. ADCs did not correlate with AQP-4 expression(P=0.416), but ADCu was significantly correlated with AQP-4 expression(P=0.034) and the correlation coefficient was 0.721. Conclusion: The ultra-high-b-value DWI can reflect the transport of AQPs.
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Received: 11 July 2017
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| Cite this article: |
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ZHANG Si-ying1,YAO Lin-peng1,WANG Qi-dong1, et al. Study on the correlation between ultra-high-b-value diffusion-weighted MR imaging and #br#
aquaporin-4 in a hepatic ischemia-reperfusion model in rats[J]. JOURNAL OF CHINA MEDICAL IMAGING, 2017, 28(10): 731-735.
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| URL: |
| http://www.jccmi.com.cn/EN/ OR http://www.jccmi.com.cn/EN/Y2017/V28/I10/731 |
[1]Jaeschke H, Lemasters JJ. Apoptosis versus oncotic necrosis in hepatic ischemia/reperfusion injury[J]. Gastroenterology, 2003, 125(4): 1246-1257.
[2]Huguet C, Addario-Chieco P, Gavelli A, et al. Technique of hepatic vascular exclusion for extensive liver resection[J]. Am J Surg, 1992, 163(6): 602-605.
[3]Delva E, Camus Y, Nordlinger B, et al. Vascular occlusions for liver resections. Operative management and tolerance to hepatic ischemia: 142 cases[J]. Ann Surg, 1989, 209(2): 211-218.
[4]Hou X, Sui W, Che W, et al. Current status and recent advances in liver transplant using organs donated after cardiac death[J]. Exp Clin Transplant, 2015, 13(1): 6-18.
[5]Jochmans I, Meurisse N, Neyrinck A, et al. Hepatic ischemia/reperfusion injury associates with acute kidney injury in liver transplantation: Prospective cohort study[J]. Liver Transpl, 2017, 23(5): 634-644.
[6]Kalisvaart M, de Haan JE, Hesselink DA, et al. The postreperfusion syndrome is associated with acute kidney injury following donation after brain death liver transplantation[J]. Transpl Int, 2017, 30(7): 660-669.
[7]Wu Y, Zhang W, Li M, et al. Nobiletin ameliorates ischemia-reperfusion injury by suppressing the function of Kupffer cells after liver transplantation in rats[J]. Biomed Pharmacother, 2017, 89: 732-741.
[8]He N, Jia JJ, Li JH, et al. Remote ischemic perconditioning prevents liver transplantation-induced ischemia/reperfusion injury in rats: Role of ROS/RNS and eNOS[J]. World J Gastroenterol, 2017, 23(5): 830-841.
[9]Vollmar B, Glasz J, Leiderer R, et al. Hepatic microcirculatory perfusion failure is a determinant of liver dysfunction in warm ischemia-reperfusion[J]. Am J Pathol, 1994, 145(6): 1421-1431.
[10]Fondevila C, Busuttil RW, Kupiec-Weglinski JW. Hepatic ischemia/reperfusion injury-a fresh look[J]. Exp Mol Pathol, 2003, 74(2): 86-93.
[11]Zhai Y, Petrowsky H, Hong JC, et al. Ischaemia-reperfusion injury in liver transplantation-from bench to bedside[J]. Nat Rev Gastroenterol Hepatol, 2013, 10(2): 79-89.
[12]Cheung JS, Fan SJ, Chow AM, et al. In vivo DTI assessment of hepatic ischemia reperfusion injury in an experimental rat model[J]. J Magn Reson Imaging, 2009, 30(4): 890-895.
[13]Xueying L, Zhongping Z, Zhoushe Z, et al. Investigation of Apparent Diffusion Coefficient from Ultra-high b-Values in Parkinson’s Disease[J]. Eur Radiol, 2015, 25(9): 2593-2600.
[14]李加慧，李秋菊，于兵，等. DWI-MRI多b值水通道蛋白分子成像机理和方法学研究[J]. 中国临床医学影像杂志，2014，25（3）：186-189.
[15]郭启勇，辛军，张新，等. MRI水扩散加权成像分子机理研究进展[J]. 中国临床医学影像杂志，2013，24（7）：496-500.
[16]Chavhan GB, Alsabban Z, Babyn PS. Diffusion-weighted imaging in pediatric body MR imaging: principles, technique, and emerging applications[J]. Radiographics, 2014, 34(3): E73-E88.
[17]Koh DM, Collins DJ. Diffusion-weighted MRI in the body: applications and challenges in oncology[J]. Am J Roentgenol, 2007, 188(6): 1622-1635.
[18]Pinker K, Helbich TH, Morris EA. The potential of multiparametric MRI of the breast[J]. Br J Radiol, 2017, 90(1069): 20160715.
[19]Rauch GM, Adrada BE, Kuerer HM, et al. Multimodality Imaging for Evaluating Response to Neoadjuvant Chemotherapy in Breast Cancer[J]. Am J Roentgenol, 2017, 208(2): 290-299.
[20]Koh DM, Collins DJ, Orton MR. Intravoxel incoherent motion in body diffusion-weighted MRI: reality and challenges[J]. Am J Roentgenol, 2011, 196(6): 1351-1361.
[21]Le Bihan D, Moonen CT, van Zijl PC, et al. Measuring random microscopic motion of water in tissues with MR imaging: a cat brain study[J]. J Comput Assist Tomogr, 1991, 15(1): 19-25.
[22]Agre P, Sasaki S, Chrispeels MJ. Aquaporins: a family of water channel proteins[J]. Am J Physiol, 1993, 265(3 Pt 2): F461.
[23]Papadopoulos MC, Verkman AS. Aquaporin-4 and brain edema[J]. Pediatr Nephrol, 2007, 22(6): 778-784.
[24]Jaeschke H, Farhood A, Smith CW. Neutrophils contribute to ischemia/reperfusion injury in rat liver in vivo[J]. FASEB J, 1990, 4(15): 3355-3359.
[25]Bjornsson B, Bojmar L, Olsson H, et al. Nitrite, a novel method to decrease ischemia/reperfusion injury in the rat liver[J]. World J Gastroenterol, 2015, 21(6): 1775-1783.
[26]Popescu ES, Pirici I, Ciurea RN, et al. Three-dimensional organ scanning reveals brain edema reduction in a rat model of stroke treated with an aquaporin 4 inhibitor[J]. Rom J Morphol Embryol, 2017, 58(1): 59-66.
[27]Masyuk AI, Larusso NF. Aquaporins in the hepatobiliary system[J]. Hepatology, 2006, 43(2 Suppl 1): S75-S81.
[28]Tietz P, Jefferson J, Pagano R, et al. Membrane microdomains in hepatocytes: potential target areas for proteins involved in canalicular bile secretion[J]. J Lipid Res, 2005, 46(7): 1426-1432. |
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