Studies on the methodology of 18F-FDG cell binding of ovarian cancer SKOV3
LIU Wen-ping1, WU Yi-wei2, ZHANG Bin2, DENG Sheng-ming2, CUI Feng1, XU Kun-yuan1
1. Department of Radiology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, China;2. Department of Nuclear Medicine, the First Hospital Affiliated to Soochow University, Suzhou Jiangsu 215006, China
Abstract：Objective: To establish a useful and stable methodology of 18F-FDG cell binding of ovarian cancer SKOV3. Methods: Several binding conditions were tested. ①The cell number was from 5×104 to 5×106 cells/bottle. ②The reaction time was from 20 to 120min. ③The radioactivity of 18F-FDG was from 1.85 to 29.6KBq. ④The glucose concentration was from 0 to 11.1mmol/L. The CPM(B) in cells and CPM(F) in supernatant were measured by the γ detector. The binding efficiency of ovarian cancer cell line SKOV3 with 18F-FDG was calculated. Results: ①There was linear regression relationship between 18F-FDG cell binding efficiency of human ovarian cancer SKOV3(y) and the cell number(x1), reaction time(x2), glucose concentration(x3). Regression equation: y=(7.395+5.18E-0.06x1+0.094x2-2.040x3)×100%, F=106.9, P<0.05. The P values of partial regression coefficient were less than 0.05. R2c=0.728, P<0.05. The Pearson correlation coefficient of cell number, reaction time, glucose concentration with 18F-FDG cell binding efficiency was 0.581、0.179、-0.599 respectively, P<0.05. ②The basic conditions of 18F-FDG cell binding of ovarian cancer SKOV3: the cell number was 1×106 cells/bottle, the radioactivity of 18F-FDG was 3.7KBq, the glucose concentration was 0mmol/L, the reaction time was 100min. The binding efficiency was (25.45±1.66)%. Conclusions: The methodology of 18F-FDG cell binding of ovarian cancer SKOV3 was established successfully. It contributed to evaluate early the effect of radiotherapy and chemotherapy on inhibiting ovarian cancer cell line SKOV3.
2011, Vol. 22 
