A preliminary study in stage Ⅰ and Ⅱ of diabetic nephropathy rats using diffusion tensor imaging MRI at 3.0T
HU Xiao-yan1, WU Ying-hua1, GUO Liang2, KUANG Min1, WANG Fan3
1． Chengdu University of TCM, Chengdu 610075, China; 2. China Medical University, Shenyang 110001, China;
3. Department of Radiology, the Affiliated Hospital of Chengdu University of TCM, Chengdu 610075, China
Abstract：Objective: To prospectively assess the manifestation of stage Ⅰ and Ⅱ diabetic nephropathy rats using diffusion tensor imaging(DTI) MRI at 3.0T. Materials and Methods: Twenty male SD rats were randomly divided into 2 groups according to the random number table. Fourteen rats were in the experimental group, the final survival of 9 rats with 18 kidneys, and 6 rats were in the control group with 12 kidneys. The experimental group was treated with 1% streptozotocin, and the control group was given the same dose of citrate buffer solution. Two weeks later, both of the two groups of rats underwent conventional MRI and DTI examination with b value of 0 and 600 s/mm2(diffusion directions=6), and the serum creatinine and microalbuminuria were measured and the pathological change of all kidneys were examined. Mean apparent diffusion coefficient(ADC) and fractional anisotropy(FA) were determined for the cortex and the medulla by two experienced radiologists blinded. Results: The blood glucose of experimental group was significantly increased, and the serum creatinine and microalbuminuria in both of the two groups of rats were normal. The pathological changes of the kidneys in the experimental group were stage Ⅰ and Ⅱ diabetic nephropathy expression, and the control group was normal. The ADC values of cortex and medulla were increased in the experimental group compared with the control group and the difference was statistically significant(P<0.05). In the experimental group, the difference of FA values were statistically significant between cortex and medulla(P<0.05). While there were no significant differences in the FA values of the cortex and medulla between two groups(P>0.05). Conclusion: The ADC value and FA value of DTI imaging maybe be more able to reflect the pathological changes than the biochemical indicator in the early stage of diabetic nephropathy.
胡小艳1，邬颖华1，郭 亮2，匡 敏1，王 钒3. Ⅰ~Ⅱ期糖尿病肾病大鼠模型扩散张量成像的初步研究[J]. 中国临床医学影像杂志, 2016, 27(11): 795-798.
HU Xiao-yan1, WU Ying-hua1, GUO Liang2, KUANG Min1, WANG Fan3. A preliminary study in stage Ⅰ and Ⅱ of diabetic nephropathy rats using diffusion tensor imaging MRI at 3.0T. JOURNAL OF CHINA MEDICAL IMAGING, 2016, 27(11): 795-798.
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