The effects of NF-κB antisense RNA gene on the levels of serum TNF-α and IL-1β in rheumatoid arthritis rats by ultrasound-mediated microbubble destruction
LIU Jie, JING Xiang-xiang, YANG Bing-ang, FU Shao-qing, WANG Dong-lin, WU Tang-na
Hainan Provincial People’s Hospital, Haikou 570311, China
Abstract：Objective: To investigate the effect of NF-κB antisense RNA gene delivery mediated by ultrasound-mediated microbubble destruction on serum levels of TNF-α, IL-1β in rheumatoid arthritis(RA) rats and its feasibility for RA therapy. Methods: The layer-by-layer assembly technique was applied to attach multiple layers of gene and polyllysine onto prepared lipid-coated microbubbles(PLL+DNA+PLL Apply+DNA)． Sixty rats were divided into six groups(10 rats with 20 knees in each group). Group A: normal control group; group B: blank control group of RA rats; group C: NF-κB antisense RNA gene plasmid group; group D: ultrasound+NF-κB antisense RNA gene plasmid group; group E: microbubbles+NF-κB antisense RNA gene plasmid group; group F: ultrasound+microbubbles+NF-κB antisense RNA gene plasmid group. The serum levels of TNF-α, IL-1β in RA rats were detected with ELISA. Results: The levels of serum TNF-α and IL-1β were obviously higher than normal control group. Those of group C to F were notably lower than that of the RA control group(P＜0.05, P＜0.01). There was no statistical difference in the levels of serum TNF-α and IL-1β among group C, D and E. But there was statistics difference between group F and group C to E(P＞0.05). Conclusion: NF-κB antisense RNA gene transfection efficiency on RA rat’s joints synovial tissues can be enhanced by ultrasound-mediated microbubble destruction, which can down-regulate the levels of serum TNF-α and IL-1β, and enhance the therapeutic effect of RA.
刘 洁，景香香，杨炳昂，符少清，王东林，吴汤娜. NF-κB反义RNA基因在超声联合微泡作用下对类风湿性关节炎大鼠血清TNF-α、IL-1β水平的影响[J]. 中国临床医学影像杂志, 2016, 27(12): 896-898.
LIU Jie, JING Xiang-xiang, YANG Bing-ang, FU Shao-qing, WANG Dong-lin, WU Tang-na. The effects of NF-κB antisense RNA gene on the levels of serum TNF-α and IL-1β in rheumatoid arthritis rats by ultrasound-mediated microbubble destruction. JOURNAL OF CHINA MEDICAL IMAGING, 2016, 27(12): 896-898.
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