大鼠肝脏缺血再灌注模型超高b值MR扩散成像与水通道蛋白-4表达的相关性研究

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摘要目的：研究水通道蛋白-4（Aquaporin-4，AQP-4）在大鼠肝脏缺血再灌注模型中的表达规律，探讨此模型超高b值磁共振扩散加权成像（Diffusion weighted imaging，DWI）与AQP-4表达的相关性。方法：选取体质量为250～300 g的SD大鼠42只，分为7组，每组6只。实验组行肝右叶门静脉和肝动脉结扎3 h后再灌注，对照组行假手术。两组分别在再灌注后0 h、6 h、12 h、1 d、3 d、7 d和14 d行3.0T MRI扫描，包括平扫T1WI和T2WI、常规DWI（0 s/mm2，1 000 s/mm2）和15个b值DWI（0～ 4 500 s/mm2）扫描，分别根据单b值DWI和15个b值DWI测量记录标准ADC值（Standard apparent diffusion coefficient，ADCs）以及超高ADC值（Ultra-high apparent diffusion coefficient，ADCu）。观察各时间点各参数的动态变化规律。扫描后给予动物安乐死，留取肝脏标本用于HE染色，光镜下观察肝脏病理形态学变化、蛋白质印迹法（Western blot）观察AQP-4表达变化。结果：实验组在术后12 h开始至3 d ADCs值均高于对照组，术后7 d开始恢复正常。实验组ADCu在术后6 h开始比对照组明显增高，术后14 d恢复正常。实验组AQP-4表达量在术后6 h、12 h、1 d、3 d逐渐降低但均高于对照组，术后7 d仍减低并开始接近正常。ADCs与AQP-4表达量不具有相关性（P=0.416），而ADCu与AQP-4表达量具有相关性（P=0.034），相关系数为0.721。结论：超高b值DWI可以反映AQP的转运情况。

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	张思影1
	尧林鹏1
	汪启东1
	洪源2
	薛星1
	孔德兴2
	陈峰1

关键词 ：肝, 再灌注损伤, 大鼠, 磁共振成像

Abstract：Objective: To study the expression of aquaporin-4(AQP-4) in hepatic ischemia-reperfusion tissue, and to explore the correlation between ultra-high-b-value magnetic resonance diffusion-weighted imaging(DWI) and the expression of AQP-4. Methods: Forty-two SD rats weighting 250～300 g were divided into 7 groups(n=6). The rats in the experimental group were treated with ligating the right lobe portal vein and hepatic artery for 3 h. The rats in the control group were treated with sham operation. 3.0T MRI scanning including plain scan T1WI, T2WI, conventional DWI(b=0 s/mm2, 1 000 s/mm2) and 15 b-values DWI(b=0～4 500 s/mm2) were performed in both groups at 0 h, 6 h, 12 h, 1 d, 3 d, 7 d and 14 d after reperfusion. Standard apparent diffusion coefficient(ADCs) and ultra-high apparent diffusion coefficient(ADCu) were measured based on single b-value DWI and 15 b-values DWI respectively. The dynamic changes of each parameter were observed at each time point. After scanning, the animals were euthanized and liver specimens were taken for HE staining. The morphological changes of the liver were noted under light microscope. The expression of AQP-4 was measured qualitatively and quantitatively by Western blot. Results: The ADCs in the experimental group were higher than those of the control group starting from 12 hours after operation and returned to normal at 7 d after operation. ADCu in the experimental group began to significantly higher than those in the control group at 6 h after operation and returned to normal at 14 d after operation. The expression of AQP-4 in the experimental group decreased gradually at 6 h, 12 h, 1 d and 3 d after operation but still higher than that in the control group and began to approach normal at 7 d after operation. ADCs did not correlate with AQP-4 expression(P=0.416), but ADCu was significantly correlated with AQP-4 expression(P=0.034) and the correlation coefficient was 0.721. Conclusion: The ultra-high-b-value DWI can reflect the transport of AQPs.

Key words： Liver Reperfusion injury Rats Magnetic resonance imaging

收稿日期: 2017-07-11

PACS:	Ｒ333.4
	Ｒ575
	Ｒ445.2

基金资助:国家自然科学基金面上项目（30670603）；浙江省卫计委培育项目（2014PYA009）。

通讯作者: 陈峰，浙江大学医学院附属第一医院放射科，310003。E-mail：chenfenghz@zju.edu.cn

作者简介: 张思影（1992-），女，辽宁本溪人，在读硕士研究生。E-mail：21518003@zju.edu.cn

引用本文:

张思影1，尧林鹏1，汪启东1，洪源2，薛星1，孔德兴2，陈峰1. 大鼠肝脏缺血再灌注模型超高b值MR扩散成像与水通道蛋白-4表达的相关性研究[J]. 中国临床医学影像杂志, 2017, 28(10): 731-735.
ZHANG Si-ying1, YAO Lin-peng1, WANG Qi-dong1, HONG Yuan2, XUE Xing1, KONG De-xing2, CHEN Feng1. Study on the correlation between ultra-high-b-value diffusion-weighted MR imaging and #br# aquaporin-4 in a hepatic ischemia-reperfusion model in rats. JOURNAL OF CHINA MEDICAL IMAGING, 2017, 28(10): 731-735.

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http://www.jccmi.com.cn/CN/ 或 http://www.jccmi.com.cn/CN/Y2017/V28/I10/731

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